Insulin Secretagogue
Insulin secretion & Beta cell function assay
A FULLY STANDARDIZED TRANSLATIONAL ASSAY VALIDATED FOR GLP-1/GIP REFERENCE COMPOUNDS
Get clinically relevant data using human primary-like pancreatic beta cells
APPLICATIONS
Insulin secretagogues and GLP-1 and GIP incretins regulate blood glucose by potentiating pancreatic insulin secretion. They now represent a treatment of reference for patients with type 2 diabetes.
EndoC-βH5® insulin secretion assays recapitulate blood glucose management in vitro taking advantages of two complementary readouts dedicated to drug screening campaigns. cAMP represents a rapid and cost-effective readout for fast drug ranking compatible with a 96 and 384 well plate for high troughput screening. Glucose Stimulated Insulin Secretion (GSIS) potentiation assay represents a more physiologically relevant golden standard for evaluation of your candidate on the insulin secretion function.
Both assays are compatible for acute and chronic treatment with a proven stability of the insulin secretion function of EndoC-βH5® cells for at least 4 weeks.
KEY ADVANTAGES
INSULIN SECRETION – 96 WELLS
cAMP – 384 WELLS
NATIVE-LIKE HUMAN BETA CELLS
ACUTE & CHRONIC TREATMENTS
DATA CONTINUITY
A highly resolutive translational assay for efficient ranking relevant clinically data
Screen your molecules with validated references compounds (Semaglutide, Tirzepatide, Exenatide)
ASSAY CONCEPT
| GSIS | cAMP | |
|---|---|---|
| Readout | Physiological | Physiological |
| Measurement | Direct | Indirect |
| Throughput | 96-wells | 384-wells |
| Cost | Medium | Low |
| Sensitivity | High | High |
| Resolution | High | High |
HAVE A PROJECT IN MIND?
Human Cell Design team of experts can offer you tailored assays and EndoC-βH5® derived cell models.
FIND THE RIGHT SERVICE FOR YOUR STUDY
Human Cell Design provides tailored services specifically designed for accelerating clinically relevant scientific progress in Type 1 and Type 2 diabetes research. From target validation to preclinical validation, our experts provide you with long term strategies and help you develop stables readouts and valuable deliverables.
